Isolation of hydrogen generating microflora from cow dung for seeding anaerobic digester

Microbial Dynamics during Anaerobic Digestion of Cow Dung

The anaerobic digestion of cow dung was investigated for 30days in a laboratory scale batch reactor. Totally nine bacterial strains were isolated from cow dung slurry. The bacterial isolates were preliminarily examined by morphological characteristics and they were further confirmed by 16S rRNA sequencing and analyzed through BLAST. Microbial dynamics showed that hydrolytic bacterial population...

Partial characterization and optimization of production of extracellular alpha-amylase from Bacillus subtilis isolated from culturable cow dung microflora.

Studies of alpha-amylase production by Bacillus subtilis (CM3) isolated earlier from cow dung microflora, were carried out. The optimum temperature, pH and incubation period for amylase production were 50-70 degrees C, 5.0-9.0 and 36 h, respectively. Enzyme secretion was very similar in the presence of any of the carbon sources tested (soluble starch, potato starch, cassava starch, wheat flour,...

Anaerobic Digester Feasibility Study

Isolation and identification of Clostridium septicum from sheep-dung

  To study the existence of Clostridium septicum as probable agent associated with currently occurrence of black quarter in Urmia area, sheep-dung samples were collected. The bacteriological examinations on suspicious gram positive anaerobic spore-bearing bacilli which are in Clostridia genus include isolation and biochemical identification were performed. The results showed 28 different types ...

Effect of Thermal Hydrolysis on Anaerobic Digester Performance

Anaerobic digestion (AD) has been recently introduced as a promising technology for producing bioenergy around the world. Due to its especial characteristics, solid waste could be considered as a proper feedstock in the digester. Retention time (RT) and rate of biogas production are two major parameters affecting the efficiency of the process. The AD procedure of biogas production consists of h...